2024-09-06
Determination of Cadmium in Food by Atomic Absorption Graphite Furnace Method
This paper establishes an analytical method for the determination of cadmium in food by atomic absorption graphite furnace method with reference to the standard “GB 5009.15-2023 National Standard for Food Safety Determination of Cadmium in Food”.
Keywords: Atomic absorption, autosampler, food, cadmium
1. Experiment Method
1.1 Instrument Configuration
Atomic Absorption Spectrophotometer AA2300 Series
Table 1 Configuration List of Atomic Absorption Spectrophotometer
| No | Modular | Qty |
| 1 | Atomic Absorption Spectrophotometer AA2310 | 1 |
| 2 | Graphite Furnace | 1 |
| 3 | Autosampler | 1 |
| 4 | Cooling Water Circulator | 1 |
| 5 | High Purity Argon | 1 |
| 6 | Graphite Tube | 1 |
1.2 Reagents and Experiment Material
1.2.1 Nitric acid solution (1+99):Pipette 10mL of nitric acid and slowly add to 990mL of water and mix well.
1.2.2 Cd Standard Solutions: 100mg/L
1.2.3 One in ten thousand analytical balances
1.2.4 Centrifuge
1.3 Sample Pretreatment
Take 0.05g sample or more (between 0.05~0.0.08), and add 10mL of 3% dilute nitric acid to the sample. Shake for 5min and centrifuge at 8000r/min for 12min. Take the supernatant and test on the machine.
2. Result and Discussion
2.1 Spectral Conditions of Cadmium
| Heating Method | Graphite Furnace Method | ||
| Testing Method | Peak Height | ||
| Injection Volume | 20μL | ||
| Spectral Bandwidth | 0.4nm | Characteristic Wavelength | 228.8nm |
| Ignite Method | AA-BG | Lamp Current | 3mA |
2.2 Standard Curve Testing and Sample Chromatogram
| Gradient Concentration Table of Standard Curve(ng/mL) | ||||
| Curve Point | 1 | 2 | 3 | 4 |
| Cadmium standard solution | 0.40 | 1.20 | 1.60 | 2.00 |
2.3 Linearity of Standard Curve
3. Conclusion
From the experimental results, the linear correlation coefficient of cadmium in the concentration range of 0.40-2.00ng/mL is greater than 0.999. The method is accurate, reliable and sensitive, and can be used for the determination of cadmium in food.
Send your inquiry directly to us
