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Determination of the Resolution Between Maltitol and Maltose by Ion Chromatography

2025-08-12

Determination of the Resolution Between Maltitol and Maltose by Ion Chromatography

Keywords: Maltitol and maltose, sugar alcohols and sugars, ion chromatography (IC), amperometric detector.

1. Experiment Method

1.1 Instrument Configuration

Table 1 Configuration List of Ion Chromatography System

No.	Modular	Qty
1	IC6300 Ion chromatograph	1
2	AS3110 Autosampler	1
3	Sugar analytical columns 4.0*250mm	1

1.2 Experiment Material and Auxiliary Equipment

Maltitol（100ug/mL）

Glucose standard solution（1000mg/L）

Sucrose standard solution（1000mg/L）

Fructose standard solution（1000mg/L）

Lactose standard solution（1000mg/L）

Maltose standard solution（1000mg/L）

The experimental water was prepared by using an ultrapure water purification system, with a resistivity of 18.25 MΩ·cm at 25°C.

1.3 Test Conditions

Instrument	Wayeal IC6300 Intelligent Ion Chromatograph
Chromatography column	Sugar analytical columns 4.0*250mm
Flow rate	1.0mL/min
Mobile phase	NaOH gradient elution
Injection volume	10μL
Column temperature	30°C	Detector temperature	30°C
Working Electrode	Au electrode	Reference electrode	Ag/AgCl
Detector	amperometric detector	Mode	Pulse Integration
Waveform selection	Gold electrode, saccharides, four-potential method

1.4 Sample Pre-treatment

Take appropriate amount of maltitol solution (100μg/mL) was diluted 10-fold, filtered through a 0.45μm aqueous syringe filter, and then analyzed by the instrument.

2. Result and Discussion

2.1 Sample Test

Figure 1. Chromatogram of a mixed standard solution for five sugars and maltitol in food

latest company case about Determination of the Resolution Between Maltitol and Maltose by Ion Chromatography 0

Figure 2. Chromatogram of a maltose standard solution

latest company case about Determination of the Resolution Between Maltitol and Maltose by Ion Chromatography 1

Fig 3. Overlaid Chromatograms of Maltitol Standard Solution for Three Injections

latest company case about Determination of the Resolution Between Maltitol and Maltose by Ion Chromatography 2

Sample Repeatability Result:

Maltitol
Retention Time RSD %	Peak Area RSD %
0.073	0.200

3. Conclusion

In this study, the resolution between maltose and maltitol was determined by using a Wayeal’s IC6300 ion chromatography system equipped with a sugar analytical column, employing NaOH gradient elution and amperometric detection.

The method is simple and fast. From the test results, it can be seen that the peak shape of maltitol is good and the response signal of the target peak is obvious. Compared with the liquid chromatography method, this method has good separation effect and high reproducibility, and is more suitable for the simultaneous detection of maltose and maltitol.

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