Determination of Sugars in Tobacco by Ion Chromatography

Determination of Sugars in Tobacco by Ion Chromatography

Water-soluble sugars mainly refer to glucose, fructose and sucrose, which are common sugars in tobacco. And they play a very important role in both the quality of tobacco and tobacco products, as well as the flavor and taste of cigarettes.

In this paper, an ion chromatography is used to determine the content of water-soluble sugar. Experimenters use IC6300 ion chromatography with an ampere detector. eluent: NaOH and sodium acetate. simple pre-treatment, with good recovery and high sensitivity, this method is suitable for the determination of water-soluble sugars.

Keywords: tobacco products; sugars; ion chromatography

1. Experiment Section

1.1 Instruments and Reagents

Wayeal IC6300 Series Ion Chromatography

Ion chromatography: Wayeal IC6300 series ion chromatography with ampere detector (Au working electrode)

Auto sampler: AS2800

Sugar column: 250mm*4.0mm

D-(+) Glucose, anhydrous (99%);

Fructose (99%);

E-(+) Sucrose, AR;

Benzoic acid (99%);

Disposable syringe (2mL)

Water system syringe filter

One ten-thousandth electronic balance

Water is prepared by the ultrapure water purifier of Wayeal with a conductivity of 18.2 MΩ - cm (25 ℃).

1.2 Instrument Parameter

Sugar column: 250mm*4.0mm

Temperature: 30℃

Detector temperature: 35 ℃

Eluent: 250mM NaOH in A; 50mM NaOH in B; 1M sodium acetate in C; pure water in D; gradient eluting;

Flow rate: 0.3mL/min

Ampere detection pulse mode: Au electrode, sugars, quaternary potential

Injection volume: 25uL

1.3 Sample Pretreatment

Flue-cured tobacco: 0.1g sample (accurate to 0.1mg) in a 250mL conical flask, add 200mL 0.1% benzoic acid solution, put the lid on and place in an ultrasonic cell for 30min, then the solution is detected on a machine after passing through a 0.22μm filter membrane.

Cigar: 0.1g sample (accurate to 0.1mg) into a 250mL conical flask, add 50mL 0.1% benzoic acid solution, put the lid on and place in an ultrasonic cell for 30min, then the solution is detected on a machine after passing through a 0.22μm filter membrane.

2. Results and Discussion

2.1 Chromatogram

A series of standard working curves of 0.1mg/L, 0.5mg/L, 1.0mg/L, 2.0mg/L, 5.0mg/L, 10.0mg/L, and 20.0mg/L are pipetted respectively. Then the multipoint overlap standard curve spectra obtained according to 1.2 working conditions as shown in Figure 1. The linear correlation coefficients of glucose, sucrose and fructose under this condition are above 0.999 with good linearity.

Figure 1 Overlapping Chromatogram of Glucose, Sucrose and Fructose

Figure 2 Standard Curve of Glucose

Figure 3 Standard Curve of Sucrose

Figure 4 Standard Curve of Fructose